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Diagnostic utility of N-terminal TMPP labels for unambiguous identification of clipped sites in therapeutic proteins
Abstract Protein therapeutics are susceptible to clipping via enzymatic and nonenzymatic mechanisms that create neo-N-termini. Typically, neo-N-termini are identified by chemical derivatization of the N-terminal amine with (N-Succinimidyloxycarbonylmethyl)tris(2,4,6-trimethoxyphenyl)phosphonium bromide (TMPP) followed by proteolysis and mass spectrometric analysis. Detection of the TMPP-labeled peptide is achieved by mapping the peptide sequence to the product ion spectrum derived from…
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Up, up, and away in Reno, Nevada
“Most people float, but I fly.” Tim Taylor, the pilot of the colorful hot air balloon my wife and I are in, shouts this at us as we ascend higher and higher, his words punctuated by bursts of burning propane propelling us skyward over the Nevada highlands. I find myself alternating between gazing at the…
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Auto show, fundraiser planned for July 15 at NASCAR Technical Institute
Special to the Tribune Speedology Lifestyle Solutions along with co-hosts, Columbia Car Meets, and title sponsor, Audi Charlotte, announce one of the most anticipated auto events of the summer for Mooresville and the surrounding areas. In their third year, these auto fundraiser events are the premier showcase of diverse vehicles and automotive innovation and it…
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Cruise Suspends All Driverless Operations Nationwide
GM’s autonomous vehicle unit Cruise is now suspending driverless operations all across America. The move comes just days after California regulators revoked Cruise’s license for driverless vehicles, declaring that Cruise’s AVs posed an “an unreasonable risk to public safety” and “are not safe for the public’s operation,” also arguing that Cruise had misrepresented information related…
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The 6 Most Haunted Places In North Dakota
Hauntings may be old hat on the Atlantic Coast of the U.S., but the cold, lonely, windswept plains of the Dakotas have an air of isolation that chills the heart. It’s hard to feel a cold draft when the air is cold to begin with. Paranormal enthusiasts have managed to find some unique places. So,…
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Everything to know about 2023 Central Florida International Auto Show: Tickets, schedule, cars, events
ORANGE COUNTY, Fla. – Hundreds of luxury, muscle, hybrid, electric, and 2024 model vehicles will be in Orlando for the 2023 Central Florida International Auto Show. The four-day event will be held November 2-5, 2023 at the Orange County Convention Center. Here’s everything you need to know about tickets, schedule, events, and what cars will…
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KCRA Weekend Roundup: Sacramento area things to do for Oct. 27-29
It’s going to be a big sports weekend with the Sacramento Kings’ home opener against the Warriors and Republic FC in their conference semifinal on Friday night. But those aren’t the only games in town, so to speak. From Halloween-themed festivals and trunk-or-treat events to the Sacramento International Auto Show, the Golden Harvest Parade, concerts…
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Cicadas Are ‘Little Hamburgers Falling From The Sky,’ Disrupting The Food Web Every 17 Years
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Auto Execs Are Coming Clean: EVs Aren’t Working
Amiga Trombone shares a report from Insider: With signs of growing inventory and slowing sales, auto industry executives admitted this week that their ambitious electric vehicle plans are in jeopardy, at least in the near term. Several C-Suite leaders at some of the biggest carmakers voiced fresh unease about the electric car market’s growth as…
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Japan’s automakers are keeping sports cars alive in the EV era
/ The Japan Mobility Show in Tokyo saw the debut of five different electrified sports cars, ranging from production-intent coupes to outrageous concept supercars. a:hover]:text-gray-63 [&>a:hover]:shadow-underline-black dark:[&>a:hover]:text-gray-bd dark:[&>a:hover]:shadow-underline-gray [&>a]:shadow-underline-gray-63 dark:[&>a]:text-gray-bd dark:[&>a]:shadow-underline-gray”>Image: Getty In auto enthusiast circles, there have been two widely shared sentiments in recent years: auto shows are dying off, and the onset of…